Rabbit polyclonal antibody to p16-INK4a (Phospho-Ser152)
Specificity
The antibody detects endogenous levels of p16-INK4a only when phosphorylated at serine 152.
Applications
WB, IHC
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 152 (G-P-S(p)-D-I) derived from Human p16-INK4a.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HeLa cells, treated with EPO (20U/ml, 15mins), using p16-INK4a (Phospho-Ser152) antibody #12148. The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p16-INK4a (Phospho-Ser152) antibody #12148. The picture on the right is treated with the synthesized peptide.
Publishing research using Anti-p16-INK4a (Phospho-Ser152) Antibody (A50378)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-p16-INK4a (Phospho-Ser152) Antibody (A50378)