Anti-p130 Cas (phospho Tyr249) Antibody (A93617)

Name: Anti-p130 Cas (phospho Tyr249) Antibody
Brand: Antibodies.com
SKU: A93617
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to p130 Cas (phospho Tyr249) for WB, IHC and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-p130 Cas (phospho Tyr249) Antibody

Description

Rabbit polyclonal antibody to p130 Cas (phospho Tyr249).

Specificity

This antibody detects endogenous levels of p130 Cas only when phosphorylated at Tyr249.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:5000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human p130 Cas around the phosphorylation site of Tyr249 (amino acids 215-264).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

93kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Breast cancer anti-estrogen resistance protein 1, CAS, Cas scaffolding protein family member 1, CASS1, CRK-associated substrate, CRKAS, p130cas

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Immunohistochemistry - Anti-p130 Cas (phospho Tyr249) Antibody (A93617)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-p130 Cas (phospho Tyr249) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-p130 Cas (phospho Tyr249) Antibody (A93617)

Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30' and A549 cells treated with PMA 125ng/ml 30' using Anti-p130 Cas (phospho Tyr249) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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