Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Otilimab Biosimilar - Anti-GM-CSF Antibody - Low endotoxin, Azide free (A323641) under reducing (R) conditions confirms that the purity of this antibody is greater than 90%.
SEC-HPLC analysis of Otilimab Biosimilar - Anti-GM-CSF Antibody - Low endotoxin, Azide free (A323641) confirms that the purity of this antibody is greater than 95%.
Immobilized recombinant human GM-CSF protein (His tag) at 2 µg/mL is bound by Otilimab Biosimilar - Anti-GM-CSF Antibody - Low endotoxin, Azide free (A323641). EC50 was approximately 0.01548 µg/ml.
Immobilized recombinant cynomolgus/rhesus macaque GM-CSF protein (hFc tag) at 2 µg/mL is bound by Otilimab Biosimilar - Anti-GM-CSF Antibody - Low endotoxin, Azide free (A323641). EC50 was approximately 0.01387 µg/ml.
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