All Lanes Mouse anti human OPN monoclonal antibody at 2 µg/mL. Lane 1 mouse kidney tissue lysate. Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size: 34.5 kDa. Observed band size: 35kDaAdditional bands at: 50 kDa. We are unsure as to the identity of this extra band.