Anti-OCT4 Antibody (A329072)

Name: Anti-OCT4 Antibody
Brand: Antibodies.com
SKU: A329072
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to OCT4 for WB, IHC-P, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

iPSCs Marker

Specifications

Name

Anti-OCT4 Antibody

Description

Rabbit polyclonal antibody to OCT4.

Applications

WB, IHC-P, ICC/IF, ELISA

Positive Control

NTERA-2.

Dilutions

WB: 1:500-1:1,000, IHC-P: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A recombinant fusion protein corresponding to amino acids 1-135 of human OCT4.

Sequence

MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

45 kDa

Predicted MW

39 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Oct-3, OCT3, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, OTF-3, OTF3, POU domain, class 5, transcription factor 1, POU5F1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-OCT4 Antibody (A329072)

OCT4 expression in NTERA-2 cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-OCT4 Antibody (A329072) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunofluorescence - Anti-OCT4 Antibody (A329072)

OCT4 expression in F9 cells analyzed by immunofluorescence. Staining was performed with Anti-OCT4 Antibody (A329072) at a dilution of 1:50 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunofluorescence - Anti-OCT4 Antibody (A329072)

OCT4 expression in NCCIT cells analyzed by immunofluorescence. Staining was performed with Anti-OCT4 Antibody (A329072) at a dilution of 1:50 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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