Recombinant Anti-Occludin Antibody [rOCLN/8525] (A315421)

Anti-Occludin Antibody [rOCLN/8525]

Recombinant mouse monoclonal [rOCLN/8525] antibody to Occludin.

Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

HepG2 cells. Human kidney.

IHC: 1-2 µg/ml

Human

Recombinant human Occludin fragment around Amino Acids 282-415. The exact sequence is proprietary.

Mouse

Monoclonal

rOCLN/8525

kappa

Protein A/G chromatography.

200 µg/ml

60-82 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

OCLN