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Anti-NUP98 Antibody [2H10] (A378)

Detection of Nup98 protein by Western blotting using this antibody, 2H10. The sample is HeLa nuclear membrane fraction. The IgG was diluted 2,000 fold before use. Immunofluorescent staining of rat neuron with antibody 2H10. The dots are Nup98. Detection of Np98 protein in whole cell extract of HeLa cells with 2H10 antibody. Immunofluorescene staining of Nup98 in HeLa cells with 2H10 antibodies. Black-and-white images were obtained with mAb and DAPI. Color images represent merged images of mAb (green) with DAPI (magenta). HeLa cells were cultured in a glass-bottom dish for 2 days before fixation. The cells were fixed with cold methanol (-30?C) for 30 min. After washing with PBS, all fixed samples were blocked with 1% BSA for 2 hr at room temperature. Anti-Nup98 rat mAb 2H10 was diluted to 0.5 µg/ml in PBS. Blocked samples were treated with these primary antibody solutions overnight at 4?C. The secondary antibodies were 4 µg/ml of Alexa488-labelled anti-rat IgG. DNA was stained with 4',6-diamidino-2-phenylindole (DAPI). Samples were washed three times with PBS between treatments. Immunofluorescene staining of Nup98 in S. pombe cells with 2H10 antibody. Detection of Nup98 in S. pombe cell extracts by Western blotting with antibodies 2H10. 1 µg/ml IgG solution of 2H10 were used. Left and right lanes represent specimens from a wild type strain and an S. pombe strain in which Nup98 was chromosomally replaced with Nup98-GFP, respectively. S. pombe cells harvested were heated in distilled water at 95°C for 5 min, suspended in 100 mM phosphate buffer (pH 6.8), 4 M urea, 2.5% SDS, 0.05 mM EDTA, and disrupted by glass beads using a Multi Beads Shocker (Yasui Kikai, Osaka,). The sample was clarified by centrifugation, and the supernatant was used as the whole cell extract. First antibody was used at 0.4 ug/ml and as second antibody, HRP-conjugated ant-rat IgG was used at 0.4 ug/ml. Numbers on the left are positions of size marker Proteins in kDa. Immunofluorescence staining of nucleoporin in S. cereviciae cell with 2H10 antibody +1
$535

Rat monoclonal (2H10) antibody to NUP98 for WB, DB, ICC/IF, ELISA and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-NUP98 Antibody [2H10]
Description
Rat monoclonal (2H10) antibody to NUP98.
Applications
WB, DB, ICC/IF, ELISA, IP
Reactivity
Human, Mouse, Rat, Schizosaccharomyces pombe
Immunogen
Recombinant human NUP98 protein (a.a. 1-466, GST tag).
Host
Rat
Clonality
Monoclonal
Clone ID
2H10
Isotype
IgG2c
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ADAR2, Nuclear pore complex protein Nup98-Nup96
Suitable Secondaries
See all Anti-Rat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (8)

Detection of Nup98 protein by Western blotting using this antibody, 2H10. The sample is HeLa nuclear membrane fraction. The IgG was diluted 2,000 fold before use.Enlarge Image

Anti-NUP98 Antibody [2H10] (A378)

Anti-NUP98 Antibody
Immunofluorescent staining of rat neuron with antibody 2H10. The dots are Nup98.Enlarge Image

Anti-NUP98 Antibody [2H10] (A378)

Anti-NUP98 Antibody
Detection of Np98 protein in whole cell extract of HeLa cells with 2H10 antibody.Enlarge Image

Anti-NUP98 Antibody [2H10] (A378)

Anti-NUP98 Antibody
Immunofluorescene staining of Nup98 in HeLa cells with 2H10 antibodies. Black-and-white images were obtained with mAb and DAPI. Color images represent merged images of mAb (green) with DAPI (magenta). HeLa cells were cultured in a glass-bottom dish for 2 days before fixation. The cells were fixed with cold methanol (-30?C) for 30 min. After washing with PBS, all fixed samples were blocked with 1% BSA for 2 hr at room temperature. Anti-Nup98 rat mAb 2H10 was diluted to 0.5 µg/ml in PBS. Blocked samples were treated with these primary antibody solutions overnight at 4?C. The secondary antibodies were 4 µg/ml of Alexa488-labelled anti-rat IgG. DNA was stained with 4',6-diamidino-2-phenylindole (DAPI). Samples were washed three times with PBS between treatments.Enlarge Image

Anti-NUP98 Antibody [2H10] (A378)

Anti-NUP98 Antibody
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