Anti-NRAS Antibody (A15785)

Name: Anti-NRAS Antibody
Brand: Antibodies.com
SKU: A15785
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to NRAS for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NRAS Antibody

Description

Rabbit polyclonal antibody to NRAS.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 90-189 of human NRAS (NP_002515.1).

Sequence

FADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVVM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

GTPase NRas, HRAS1, Transforming protein N-Ras

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-NRAS Antibody (A15785)

Western blot analysis of extracts of various cell lines, using Anti-NRAS Antibody (A15785) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 10s.

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Western Blot - Anti-NRAS Antibody (A15785)

Western blot analysis of extracts of Mouse brain, using Anti-NRAS Antibody (A15785) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunofluorescence - Anti-NRAS Antibody (A15785)

Immunofluorescence analysis of C6 cells using Anti-NRAS Antibody (A15785) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-NRAS Antibody (A15785)

Immunofluorescence analysis of NIH/3T3 cells using Anti-NRAS Antibody (A15785) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).