Anti-NQO1 Antibody [ARC56753] (A307961)

Western blot analysis of extracts of various lysates, using Anti-NQO1 Antibody [ARC56753] (A307961) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts from wild type(WT) and NQO1 knockdown (KD) HeLa cells, using Anti-NQO1 Antibody [ARC56753] (A307961) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-NQO1 Antibody [ARC56753] (A307961) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat stomach using Anti-NQO1 Antibody [ARC56753] (A307961) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).