NQO1 expression in U251 (A) and HepG2 (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-NQO1 Antibody (A82457) at 0.3µg/ml and detected by chemiluminescence.
NQO1 expression in Human Kidney (A), Lung (B) and Duodenum (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-NQO1 Antibody (A82457) at 0.3µg/ml and detected by chemiluminescence.
NQO1 expression in Rat Kidney (A), Lung (B) and Duodenum (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-NQO1 Antibody (A82457) at 0.3µg/ml and detected by chemiluminescence.
NQO1 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-NQO1 Antibody (A82457) at 2µg/ml and revealed with alkaline phosphatase (AP).
NQO1 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NQO1 Antibody (A82457) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and some nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
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