Anti-NQO1 Antibody (A12506)

Name: Anti-NQO1 Antibody
Brand: Antibodies.com
SKU: A12506
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to NQO1 for WB, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NQO1 Antibody

Description

Rabbit polyclonal antibody to NQO1.

Applications

WB, ICC/IF, ELISA

Positive Control

HepG2, MCF7, HeLa, Mouse liver.

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 1-274 of human NQO1.

Sequence

MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

31 kDa

Predicted MW

31 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Azoreductase, DIA4, DT-diaphorase, DTD, Menadione reductase, NAD(P)H dehydrogenase [quinone] 1, NAD(P)H:quinone oxidoreductase 1, NMOR1, Phylloquinone reductase, QR1, Quinone reductase 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-NQO1 Antibody (A12506)

NQO1 expression in HeLa cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-NQO1 Antibody (A12506) at a dilution of 1:3000 and detected with chemiluminescence.

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Western Blot - Anti-NQO1 Antibody (A12506)

NQO1 expression in Mouse liver lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-NQO1 Antibody (A12506) at a dilution of 1:400 and detected with chemiluminescence.

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Western Blot - Anti-NQO1 Antibody (A12506)

NQO1 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-NQO1 Antibody (A12506) at a dilution of 1:500 and detected with chemiluminescence.

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Immunofluorescence - Anti-NQO1 Antibody (A12506)

NQO1 expression in NIH/3T3 cells analyzed by immunofluorescence. Staining was performed with Anti-NQO1 Antibody (A12506) at a dilution of 1:50 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).