Western Blot - Anti-NMDAR1 (phospho Ser890) Antibody (A88231)
Western blot analysis of extracts of C6 cells, using Anti-NMDAR1 (phospho Ser890) Antibody (A88231) at 1:1,000 dilution. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 90s.
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