Anti-NLRP3 Antibody (A307942)

Name: Anti-NLRP3 Antibody
Brand: Antibodies.com
SKU: A307942
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to NLRP3 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-NLRP3 Antibody

Description

Rabbit polyclonal antibody to NLRP3.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of mouse NLRP3 (NP_665826.1).

Sequence

MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNARLGESVDLNSRYTQLQLVKEHPSKQEREHELLTIGRTKMRDSPMSSLKLELLFEPEDGHSEPVHTVVFQGAAGIGKTILARKIMLDWALGKLFKDKFDYLFFIHCREVSLRTPRSLADLIVSCWPDPNPPVCKILRKPSRILFLMDGFDELQGAFDEHIGEVCTDWQKAVRGDILLSSLIRKKLLPKASLLITTRPVALEKLQHLLDH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, Caterpiller protein 1.1, CIAS1, CLR1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, NACHT, LRR and PYD domains-containing protein 3, NALP3, PYPAF1, PYRIN-containing APAF1-like protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-NLRP3 Antibody (A307942)

Western blot analysis of RAW264. 7, using Anti-NLRP3 Antibody (A307942) at 1:700 dilution. Raw264. 7 cells were treated by LPS (1 µg/ml) at 37°C for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunofluorescence - Anti-NLRP3 Antibody (A307942)

Immunofluorescence analysis of HepG2 cells using Anti-NLRP3 Antibody (A307942) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-NLRP3 Antibody (A307942)

Immunofluorescence analysis of NIH/3T3 cells using Anti-NLRP3 Antibody (A307942) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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