Anti-NLRP1 Antibody (A91527)

Name: Anti-NLRP1 Antibody
Brand: Antibodies.com
SKU: A91527
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to NLRP1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NLRP1 Antibody

Description

Rabbit polyclonal antibody to NLRP1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1370-1473 of human NLRP1 (NP_127497.1).

Sequence

PSPLDAPQLLHFVDQYREQLIARVTSVEVVLDKLHGQVLSQEQYERVLAENTRPSQMRKLFSLSQSWDRKCKDGLYQALKETHPHLIMELWEKGSKKGLLPLSS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CARD7, Caspase recruitment domain-containing protein 7, Death effector filament-forming ced-4-like apoptosis protein, DEFCAP, KIAA0926, NAC, NACHT, LRR and PYD domains-containing protein 1, NACHT, LRR and PYD domains-containing protein 10, NALP1, NALP10, NLRP10, NOD8, Nucleotide-binding domain and caspase recruitment domain, Nucleotide-binding oligomerization domain protein 8, PYNOD

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-NLRP1 Antibody (A91527)

Western blot analysis of extracts of K-562 cells, using Anti-NLRP1 Antibody (A91527) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunohistochemistry - Anti-NLRP1 Antibody (A91527)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-NLRP1 Antibody (A91527) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-NLRP1 Antibody (A91527)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-NLRP1 Antibody (A91527) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-NLRP1 Antibody (A91527)

Immunofluorescence analysis of K-562 cells using Anti-NLRP1 Antibody (A91527) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).