Anti-NLRC4 Antibody (A329679)

Name: Anti-NLRC4 Antibody
Brand: Antibodies.com
SKU: A329679
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to NLRC4 for WB, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NLRC4 Antibody

Description

Rabbit polyclonal antibody to NLRC4.

Applications

WB, ICC/IF, ELISA

Positive Control

Mouse large intestine.

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 71-170 of mouse NLRC4.

Sequence

KSLENWDYFVYQDLTGQNLSYQVTEEDLNVLAQNLKDLYNSPAFLNFYPLGEDIDIIFNLEKTFTEPIMWKKDHRHHRVEQLTLGSLLEALKSPCLIEGE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

117 kDa

Predicted MW

116 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CARD, LRR, and NACHT-containing protein, CARD12, Caspase recruitment domain-containing protein 12, CED-4-like protein Clan, CLAN, CLAN1, Ice protease-activating factor, IPAF, NLR family CARD domain-containing protein 4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-NLRC4 Antibody (A329679)

NLRC4 expression in Mouse large intestine lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-NLRC4 Antibody (A329679) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunofluorescence - Anti-NLRC4 Antibody (A329679)

NLRC4 expression in Human spleen tissue analyzed by immunofluorescence. Staining was performed with Anti-NLRC4 Antibody (A329679) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunofluorescence - Anti-NLRC4 Antibody (A329679)

NLRC4 expression in Jurkat cells analyzed by immunofluorescence. Staining was performed with Anti-NLRC4 Antibody (A329679) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunofluorescence - Anti-NLRC4 Antibody (A329679)

NLRC4 expression in PC-12 cells analyzed by immunofluorescence. Staining was performed with Anti-NLRC4 Antibody (A329679) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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