Rabbit polyclonal antibody to NIFK (phospho Thr234).
Specificity
This antibody detects endogenous levels of NIFK only when phosphorylated at Thr234.
Applications
IHC, IF, ELISA
Dilutions
IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:40000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human NIFK around the phosphorylation site of Thr234 (amino acids 200-249).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
34kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hNIFK, MKI67 FHA domain-interacting nucleolar phosphoprotein, MKI67IP, NOPP34, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67
Immunohistochemical analysis of paraffin-embedded human brain using Anti-NIFK (phospho Thr234) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence analysis of HUVEC cells using Anti-NIFK (phospho Thr234) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human brain using Anti-NIFK (phospho Thr234) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
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