Western blot analysis of rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Anti-NFATC1 Antibody (A305167) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.
Immunohistochemistry analysis of human spleen, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-NFATC1 Antibody (A305167) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X.
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