Anti-NFAT2 Antibody (A329671)

Name: Anti-NFAT2 Antibody
Brand: Antibodies.com
SKU: A329671
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to NFAT2 for WB, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NFAT2 Antibody

Description

Rabbit polyclonal antibody to NFAT2.

Applications

WB, ICC/IF, ELISA

Positive Control

Mouse thymus, Mouse spleen, Daudi, Ramos.

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant protein corresponding to amino acids 595-694 of human NFAT2.

Sequence

LPLVEKQSTDSYPVVGGKKMVLSGHNFLQDSKVIFVEKAPDGHHVWEMEAKTDRDLCKPNSLVVEIPPFRNQRITSPVHVSFYVCNGKRKRSQYQRFTYLP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

70 kDa

Predicted MW

38 kDa, 74-88 kDa, 100-101 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

NF-ATc, NF-ATc1, NFAT transcription complex cytosolic component, NFATc, NFATc1, Nuclear factor of activated T-cells, cytoplasmic 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-NFAT2 Antibody (A329671)

NFAT2 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-NFAT2 Antibody (A329671) at a dilution of 1:400 and detected with chemiluminescence.

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Western Blot - Anti-NFAT2 Antibody (A329671)

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Immunofluorescence - Anti-NFAT2 Antibody (A329671)

NFAT2 expression in U2OS cells analyzed by immunofluorescence. Staining was performed with Anti-NFAT2 Antibody (A329671) at a dilution of 1:25 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunofluorescence - Anti-NFAT2 Antibody (A329671)

NFAT2 expression in THP-1 cells analyzed by immunofluorescence. Staining was performed with Anti-NFAT2 Antibody (A329671) at a dilution of 1:25 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).