Anti-NF-M Antibody [3H11] (A85325)

Immunofluorescence of a section of spinal cord from a rat given a C4 contusion injury 3 days previously. The section was stained with Anti-NF-M Antibody [3H11] (A85325) (green) and counterstained with Anti-NF L Antibody (A333279) at a dilution of 1:1,000 (red). The Anti-NF L Antibody (A333279) does not stain the undamaged axons which are strongly positive for the NF-M antibody. However, linear arrays of swollen profiles which originated from damaged axons are strongly positive for the Anti-NF L Antibody (A333279) antibody but not the NF-M antibody, although there is clearly some staining. The Anti-NF-M Antibody [3H11] (A85325) epitope, which is in the C-terminal “tail” of NF-M, has either been partially removed or destroyed.

Immunohistochemistry analysis of a 4% PFA fixed paraffin embedded rat cerebellum section with Anti-NF-M Antibody [3H11] (A85325) at a dilution of 1:10,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed tissues but does not stain long term NBF fixed tissue effectively.