Anti-NF-M Antibody [3H11] (A85325)

Immunofluorescence analysis of adult rat frontal cortex section stained with Anti-NF-M Antibody (1:5,000 | green) and Anti-NF-H Antibody (A85337 | 1:5,000 | red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. Anti-NF-M Antibody labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the Anti-NF-H Antibody stains the network of neuronal axons only.

Culture of adult neural cells. Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with Anti-Alpha-Internexin Antibody (A85441). These are mitotic neuronal progenitor cells and express many other neuronal markers.

Western blot analysis of neuronal tissue lysates using Anti-NF-M Antibody (1:10,000 | green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (Lane 1). The remaining lanes show blots of this material stained with various antibodies including Anti-NF-M Antibody (Lane 3).