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Anti-NF-kB p65 (phospho Ser529) Antibody [ARC65114] (A329673)

Western Blot - Anti-NF-kB p65 (Phospho S529) Antibody [ARC65114] (A329673) - Antibodies.com
$485

Rabbit monoclonal [ARC65114] antibody to NF-kB p65 (phospho Ser529) for WB and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-NF-kB p65 (phospho Ser529) Antibody [ARC65114]
Description
Rabbit monoclonal [ARC65114] antibody to NF-kB p65 (phospho Ser529).
Applications
WB, ELISA
Positive Control
HeLa treated by TNF-a and Calyculin A.
Dilutions
WB: 1:500-1:1,000
Reactivity
Human
Immunogen
A synthetic peptide phosphorylated at S529 of human NF-kB p65.
Sequence
LLSGD
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC65114
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
65 kDa
Predicted MW
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 0.05% BSA, 50% Glycerol, and 0.05% ProClin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
NFKB3, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-NF-kB p65 (Phospho S529) Antibody [ARC65114] (A329673) - Antibodies.comEnlarge Image

Western Blot - Anti-NF-kB p65 (phospho Ser529) Antibody [ARC65114] (A329673)

NF-kB p65 expression in HeLa cell lysates analyzed by western blot. Cells were treated with TNF-a (100 ng/ml) and Calyculin A (50 nM) at 37°C for 30 minutes. Primary antibody incubation was performed for 1 hour on 45ug protein per lane with Anti-NF-kB p65 (Phospho S529) Antibody [ARC65114] (A329673) at a dilution of 1:1000 and detected with chemiluminescence.
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