Western Blot - Anti-NF-kB p65 (phospho Ser276) Antibody (A16452)
Western blot analysis of HeLa, using Anti-NF-kB p65 (phospho Ser276) Antibody (A16452) at 1:400 dilution. HeLa cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Publishing research using Anti-NF-kB p65 (phospho Ser276) Antibody (A16452)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-NF-kB p65 (phospho Ser276) Antibody (A16452)
![Western Blot - Anti-NF-kB p65 Antibody [ARC51086] (A306573) - Antibodies.com](https://cdn.antibodies.com/image/catalog/306/A306573_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-NF-kB p65 Antibody [PCRP-RELA-2B6] - BSA and Azide free (A253058) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253058_1.jpg?profile=product_alternative)
