Anti-NF-kB p65 Antibody (A80443)

Name: Anti-NF-kB p65 Antibody
Brand: Antibodies.com
SKU: A80443
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to NF-kB p65 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-NF-kB p65 Antibody

Description

Rabbit polyclonal antibody to NF-kB p65.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-kB p65/RelA (NP_068810.3).

Sequence

LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

NFKB3, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-NF-kB p65 Antibody (A80443)

Western blot analysis of extracts of various cell lines, using Anti-NF-kB p65 Antibody (A80443) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-NF-kB p65 Antibody (A80443)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-NF-kB p65 Antibody (A80443) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-NF-kB p65 Antibody (A80443)

Immunofluorescence analysis of NIH/3T3 cells using Anti-NF-kB p65 Antibody (A80443) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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