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Anti-NF-kB p65 Antibody (A283785)

$780

Rabbit polyclonal antibody to NF-kB p65 for WB, ELISA, IF, IP and Gel Super Shift Assays.

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Anti-NF-kB p65 Antibody
Description
Rabbit polyclonal antibody to NF-kB p65.
Specificity
This antibody recognises the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NF?B p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NF?B p65 is a 551 amino acid ~65 kDa subunit of the NF?B transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NF?B appears to be the p65-p50 heterodimer. In the active form NF?B p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NF?B p65 is also found in an inactive form in the cytoplasm associated with the inhibitor I?B (UniProt: Q04206) In a gel supershift assay Rabbit anti Human NF?B p65 antibody was found to be active against all p65 containing human, mouse or rat NF?B complexes using 0.5ul to 1.0ul per assay. The binding of Rabbit anti Human NF?B p65 antibody to the NF?B p65 subunit can be blocked using the NF?B p65 control peptide (PHP073).
Applications
WB, ELISA, IF, IP, Gel Super Shift Assays
Dilutions
ELISA: 1:5,000 - 1:25,000, Gel Super Shift Assays: 0.5 - 1 ul per test, WB: 1:2,000 - 1:5,000
Reactivity
Human, Mouse, Rat
Immunogen
NFkB p65 peptide corresponding to the C-terminus region of the human protein conjugated to Keyhole Limpet Haemocyanin.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Concentration
Lot Specific
Purity
Serum.
Product Form
Liquid
Formulation
Supplied as serum with 0.01% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Human NFkB p65 antibody recognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NF?B p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NF?B p65 is a 551 amino acid ~65 kDa subunit of the NF?B transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NF?B appears to be the p65-p50 heterodimer. In the active form NF?B p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NF?B p65 is also found in an inactive form in the cytoplasm associated with the inhibitor I?B (UniProt: Q04206) In a gel supershift assay Rabbit anti Human NF?B p65 antibody was found to be active against all p65 containing human, mouse or rat NF?B complexes using 0.5ul to 1.0ul per assay. The binding of Rabbit anti Human NF?B p65 antibody to the NF?B p65 subunit can be blocked using the NF?B p65 control peptide (PHP073).
Synonyms
NFKB3, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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