Anti-NF-H Antibody [AH1] (A85340)

Immunohistological analysis of rat cerebellum section stained with Anti-Neurofilament Heavy Polypeptide Antibody [AH1] (A85340), dilution 1:2,000, in green, and co-stained with Anti-Fox3 Antibody (A85403), dilution 1:5,000, in red. Following transcardial perfusion with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. The Anti-Neurofilament Heavy Polypeptide Antibody [AH1] stains axons in the granular layer and white matter and prominent basket cell axons surrounding the large Purkinje neurons. The FOX3 antibody specifically labels nuclei of granular and other neurons, but does not stain Purkinje cells.

Western blot detection of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20µg/lane) with Anti-Neurofilament Heavy Polypeptide Antibody [AH1] (A85340) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200 kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.