Anti-NF-H Antibody (A85336)

Immunohistological analysis of a mouse hippocampus section stained with Anti-NF-H Antibody, at a dilution of 1:2,000, in red, and Anti-MBP Antibody (A85322 | 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-NF-H Antibody labels a network of axons of different neurons, while the Anti-MBP Antibody stains myelin sheath around these axons.

Mixed neuron/glia cultures stained with Anti-NF-H Antibody (green) and Anti-NF-L Antibody (A85286 | red). Axons contain phosphorylated NF-H and NF-L so appear yellow, while dendrites and perikarya only contain NF-L and so appear red. DNA is shown in blue.

Western blot analysis of different tissue lysates using Anti-NF-H Antibody, at a dilution of 1:10,000, in green,: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord [Lane 4] mouse brain, and [Lane 5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected with Anti-NF-H Antibody.

Immunofluorescent analysis of rat brain stem section stained with Anti-Myelin Basic Protein Antibody [7G7] (A85322) at a dilution of 1:5,000 (green) and costained with Anti-NF-H Antibody (A85336) at a dilution of 1:2,000 (red). This antibody stains oligodendrocytes and the myelin sheathes around axons. In this high magnification view it is clear that the NF-H antibody labels axons within the myelin sheathes.