Anti-NF-H Antibody (A270594)

Immunofluorescence analysis of mouse cerebellum section stained with Anti-Neurofilament Heavy Polypeptide Antibody (A270594), dilution 1:3,000, in red, and co-stained with Anti-MBP Antibody (A85322), dilution 1:5,000, in green. Following transcardial perfusion with 4% paraformaldehyde, the mouse brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with above antibodies. The NF-H antibody labels axons of basket and Purkinje cells and others. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons in the granular layer at center and the white matter at bottom left.

Western blot analysis of tissue lysates from different species using Anti-Neurofilament Heavy Polypeptide Antibody (A270594), dilution 1:20,000, in green. The lanes contain: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, [5] cow spinal cord, [6] pig hippocampus and [7] pig spinal cord. The strong band at about 220 kDa corresponds to the major phospho-NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.