Anti-Neurofilament Heavy Polypeptide Antibody [NE14] (A249491)

Anti-Neurofilament Heavy Polypeptide Antibody [NE14]

Mouse monoclonal [NE14] antibody to Neurofilament Heavy Polypeptide.

This antibody reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

WB, Flow Cytometry, IHC-P

WB: 1-2 µg/ml, Flow Cytometry: 1-2 µg/million cells, IHC-P: 0.25-0.5 µg/ml

Human, Mouse, Rat, Guinea Pig, Gerbil, Feline, Porcine, Rabbit, Bovine, Chicken

Crude neurofilament preparation from porcine spinal cord.

Mouse

Monoclonal

NE14

IgG1

Protein A/G chromatography.

200 µg/ml

200 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Neurofilament Heavy Polypeptide Antibody [NE14] - BSA and Azide free (A252671).

200 kDa neurofilament protein, KIAA0845, NEFH, Neurofilament triplet H protein, NFH