Anti-NeuN Antibody [NEUN/7167] (A315387)

Anti-NeuN Antibody [NEUN/7167]

Mouse monoclonal [NEUN/7167] antibody to NeuN.

NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Human brain. HeLa cells.

IHC: 1-2 µg/ml

Human

Recombinant fragment (around Amino Acids 1-200) of human RBFOX3 (NeuN) protein. The exact sequence is proprietary.

Mouse

Monoclonal

IgG2b

kappa

Protein A/G chromatography.

200 µg/ml

48 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Fox-1 homolog C, NeuN antigen, Neuronal nuclei antigen, RBFOX3, RNA binding protein fox-1 homolog 3