Anti-NeuN Antibody (A85403)

Western blot of rat brain homogenates stained with Anti-NeuN Antibody. Antibody binds closely spaced 48 and 46kDa bands.

Immunofluorescent analysis of a rat brain section stained with Anti-NeuN Antibody (A85403), at a dilution of 1:2,000, in red, and co-stained with Anti-MAP2 Antibody (A85363), at a dilution of 1:5,000, in green. Following transcardial perfusion of a rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. The Anti-NeuN Antibody (A85403) selectively stains nuclei and cytoplasm of neuronal cells, while the Anti-MAP2 Antibody (A85363) labels dendrites and overlaps with Fox3 staining in neuronal perikarya.

Immunofluorescent analysis of a rat frontal cortex section stained with Anti-NF-L Antibody [6H112] (A270560) at a dilution of 1:2,000 (green) and costained with Anti-NeuN Antibody (A85403) at a dilution of 1:2,000 (red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-NF-L Antibody [6H112] (A270560) labels the cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the FOX3/NeuN antibody selectively stains nuclei and cytoplasm of neuronal cells.

Immunohistochemistry analysis of a formalin fixed paraffin embedded mouse hippocampus section with Anti-NeuN Antibody (A85403) at a dilution of 1:4,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. The Anti-NeuN Antibody (A85403) selectively labels the nuclei and distal perikaryal of most neuronal cell populations. Note: this antibody performs well in testing with 4% PFA and NBF fixed mouse, human, and rat tissues.