Anti-NeuN Antibody [1B7] (A85405)

Blots of crude rat brain extract stained with Anti-NeuN Antibody. NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48 kDa.

Western blot analysis of whole brain tissue lysates using Anti-NeuN Antibody [1B7] (A85405), dilution 1:1,000, in green. The lanes contain: [Lane 1] protein standards (red), [Lane 2] adult rat brain, [Lane 3] embryonic E20 rat brain, [Lane 4] adult mouse brain. Note the strong twin bands corresponding to the two alternate transcripts of the NeuN protein with apparent SDS-PAGE molecular weights of 46 and 48 kDa. As with other NeuN antibodies, an additional band at ~70 kDa is revealed in some lysates.

Binding curve set for Anti-NeuN Antibody [1B7] (A85405) with 25nM IgG and limiting dilutions of recombinant FOX3 peptide (0-500nM) obtained using label-free bio-layer interferometry system (Octet RED96). Color-coded traces show sensorgram data normalized to baseline after subtraction of 0nM IgG signal from all channels. Traces with overlying fit lines in red indicate their inclusion in the global fit analysis used to derive kinetic parameters listed under the legend (R^2 – goodness of correlation between the fit and data; kon – association rate constant; koff – dissociation rate constant; KD = koff/kon – affinity constant/equilibrium dissociation constant).

Immunohistochemistry analysis of a formalin fixed paraffin embedded rat hippocampus section with mouse mAb to FOX3/NeuN, 1mg/mL Anti-NeuN Antibody [1B7] (A85405) at a dilution of 1:2,000 detected in DAB (brown) following the Vector Labs ImmPRESS method with citra retrieval. Counterstained with Hematoxylin (blue). This antibody has also been validated on NBF fixed human tissues.