Anti-NeuN Antibody [1B7] (A85405)

Immunofluorescent analysis of rat brain stem co-stained with Anti-NeuN Antibody [1B7] (A85405) in green, and Anti-MAP2 Antibody (A85363) in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. The NeuN antibody selectively stains nuclei and the proximal cytoplasm of neuronal cells while the MAP2 antibody labels dendrites and overlaps with NeuN staining in the perikarya of neurons.

Western blot analysis of whole brain tissue lysates using Anti-NeuN Antibody [1B7] (A85405), dilution 1:1,000, in green. The lanes contain: [1] protein standards (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Note the strong twin bands corresponding to the two alternate transcripts of the NeuN protein with apparent SDS-PAGE molecular weights of 46 and 48 kDa. As with other NeuN antibodies, an additional band at ~70 kDa is revealed in some lysates.