Immunofluorescent analysis of rat embryonic (E18) brain stained with Anti-Nestin Antibody (1:2,000 | red). Blue is Hoechst staining of nuclear DNA. Anti-Nestin Antibody produces strong staining of the developing radial glia and astrocytes and their processes.
Western blot analysis analysis of tissue and cell lysates using Anti-Nestin Antibody (1:5,000 | green): [1] protein standard, [2] embryonic day 18 rat brain, [3] rat cortical neuron-glial cell culture, [4] C6 and [5] SH-SY5Y cells. The high molecular weight bands correspond to nestin protein.
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