Recombinant humanized monoclonal antibody to IL-31RA.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Cynomolgus Macaque
Host
Humanized
Clonality
Monoclonal
Isotype
IgG2SA - Human IgG2 with S387A mutation
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 144.92 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Nemolizumab Biosimilar - Anti-IL-31RA Antibody - Low endotoxin, Azide free (A323603) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Nemolizumab Biosimilar - Anti-IL-31RA Antibody - Low endotoxin, Azide free (A323603) confirms that the purity of this antibody is 100%.
Human IL31R HEK293 cells were stained with Nemolizumab Biosimilar - Anti-IL-31RA Antibody - Low endotoxin, Azide free (A323603) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.307 µg/ml.
Nemolizumab Biosimilar - Anti-IL-31RA Antibody - Low endotoxin, Azide free (A323603) P-STAT3 Assay was evaluated using Du145. The IC50 was approximately 0.1068 µg/ml.
Nemolizumab Biosimilar - Anti-IL-31RA Antibody - Low endotoxin, Azide free (A323603) activation inhibition was evaluated using Hacat. The max induction fold was approximately 1.86.
Publishing research using Nemolizumab Biosimilar - Anti-IL-31RA Antibody - Low endotoxin, Azide free (A323603)? Please let us know so that we can list the citation on this page.
Alternative products to Nemolizumab Biosimilar - Anti-IL-31RA Antibody - Low endotoxin, Azide free (A323603)
