Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free
Description
Recombinant chimeric monoclonal [DMC438] antibody to Nectin 4.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit / Human
Clonality
Monoclonal
Clone ID
DMC438
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.
Flow cytometry analysis of Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) on Expi293 cells transfected with human Nectin4 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740). (A) Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) does not bind to 293T cells that do not express Nectin4. (B) A clear peak shift of Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) was seen compared to the control when incubated with Nectin4-expressing A431 cells, indicating strong binding of Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) to Nectin4. Antibodies were incubated at 2 µg/ml.
Publishing research using Recombinant Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740)? Please let us know so that we can list the citation on this page.
![Flow Cytometry - Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318740_1.jpg?profile=product_top)
![Flow Cytometry - Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318740_2.jpg?profile=product_top)
![Flow Cytometry - Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318740_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318740_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318740_1.jpg?profile=product_image)
![Flow Cytometry - Anti-Nectin 4 Antibody [DMC438] - BSA and Azide free (A318740) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318740_2.jpg?profile=product_image)