Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Necitumumab Biosimilar - Anti-EGFR Antibody - Low endotoxin, Azide free (A323601) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Necitumumab Biosimilar - Anti-EGFR Antibody - Low endotoxin, Azide free (A323601) confirms that the purity of this antibody is greater than 95%.
Human EGFR CHO-K cells were stained with Necitumumab Biosimilar - Anti-EGFR Antibody - Low endotoxin, Azide free (A323601) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.08413 µg/ml.
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