Anti-Ndufs1 Antibody (A92653)

Name: Anti-Ndufs1 Antibody
Brand: Antibodies.com
SKU: A92653
Price: 355.0 USD
Availability: InStock

Enlarge Image

$355

Rabbit polyclonal antibody to Ndufs1 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Ndufs1 Antibody

Description

Rabbit polyclonal antibody to Ndufs1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-290 of human NDUFS1 (NP_004997.4).

Sequence

VEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CI-75kD, Complex I-75kD, NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

Enlarge Image

Western Blot - Anti-Ndufs1 Antibody (A92653)

Western blot analysis of extracts of various cell lines, using Anti-Ndufs1 Antibody (A92653) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Immunohistochemistry - Anti-Ndufs1 Antibody (A92653)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Ndufs1 Antibody (A92653) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Immunofluorescence - Anti-Ndufs1 Antibody (A92653)

Immunofluorescence analysis of HeLa cells using Anti-Ndufs1 Antibody (A92653) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Immunofluorescence - Anti-Ndufs1 Antibody (A92653)

Immunofluorescence analysis of HepG2 cells using Anti-Ndufs1 Antibody (A92653) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).