Western Blot - Anti-NDRG1 (phospho Thr346) Antibody (A90200)
Western blot analysis of extracts of C6 cells, using Anti-NDRG1 (phospho Thr346) Antibody (A90200) at 1:1,000 dilution. C6 cells were treated by IGF-1 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Publishing research using Anti-NDRG1 (phospho Thr346) Antibody (A90200)? Please let us know so that we can list the citation on this page.