Western blot analysis of extracts of NIH/3T3 cells, using Anti-Nanog Antibody (A9165) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded human testicular seminoma using Anti-Nanog Antibody (A9165) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-Nanog Antibody (A9165)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-Nanog Antibody (A9165)