Anti-Nanog Antibody (A84315)

Name: Anti-Nanog Antibody
Brand: Antibodies.com
SKU: A84315
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to Nanog for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 3-5 business days.

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iPSCs Marker

Specifications

Name

Anti-Nanog Antibody

Description

Goat polyclonal antibody to Nanog.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:128,000, WB: 0.05-0.1µg/ml, IHC: 1-2µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human Nanog.

Sequence

C-DLFLNYSMNMQPED

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

35 kDa

Predicted MW

34.6kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Nanog Antibody (A84315)

Nanog expression in HeLa nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Nanog Antibody (A84315) at 0.05µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-Nanog Antibody (A84315)

Nanog expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Nanog Antibody (A84315) at 2µg/ml and revealed with alkaline phosphatase (AP).

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