Anti-Myelin Basic Protein Antibody [MBP/4275] (A315350)

Anti-Myelin Basic Protein Antibody [MBP/4275]

Mouse monoclonal [MBP/4275] antibody to Myelin Basic Protein.

Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4275 is used in clinical diagnostics to detect MBP levels or myelination in human MBP. MBP/4275 is used in a two-site ELISA with clone MBP/4273. Both are used interchangeably as capture and detection antibody.

ELISA, ICC, WB, IF, IHC-P

Human brain or astrocytoma tissue.

ICC: 0.5-1 µg/ml, WB: 1-2 µg/ml, IF: 1-2 µg/ml, IHC: 1-2 µg/ml

Human, Chicken, Guinea Pig, Rat, Porcine, Rabbit, Sheep

Spleen cells from an immunised outbred rat were fused with cells of the mouse NS0 myeloma cell line.

Mouse

Monoclonal

IgG

Protein A/G chromatography.

200 µg/ml

13-21 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein