Western blot analysis of extracts of various cell lines, using MCCC2 antibody (A7990) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Western blot analysis of extracts of various cells, using MCCC2 antibody (A7990) at 1:1,000 dilution ratio through one-step method. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:4000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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