Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Anti-Myc Tag Antibody (A17324) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-Myc Tag Antibody (A17324)
Immunofluorescence analysis of HeLa-Myc-CandHeLa cells using Anti-Myc Tag Antibody (A17324) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-Myc Tag Antibody (A17324). This Western blot was performed on the immunoprecipitate using Anti-Myc Tag Antibody (A17324) at a dilution of 1:50000.
Immunoprecipitation analysis of 300µg extracts of 293T-NLK-Myc cells using 3µg of Anti-Myc Tag Antibody (A17324). This Western blot was performed on the immunoprecipitate using Anti-Myc Tag Antibody (A17324) at a dilution of 1:20000.
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