Anti-MUC1 Antibody [SPM492] (A249409)

Anti-MUC1 Antibody [SPM492]

Mouse monoclonal [SPM492] antibody to MUC1.

This antibody reacts with MUC1. The dominant epitope of This antibody has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

ELISA, Flow Cytometry, IF, IHC-P

ELISA: 2-4 µg/ml, Flow Cytometry: 0.25-0.5 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Mouse

Breast Cancer Cell line ZR75-1.

Mouse

Monoclonal

SPM492

IgG1

Protein A/G chromatography.

200 µg/ml

265-400 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-MUC1 Antibody [SPM492] - BSA and Azide free (A252589).

Breast carcinoma-associated antigen DF3, CA 15-3, Cancer antigen 15-3, Carcinoma-associated mucin, CD227, Episialin, H23AG, MUC-1, Mucin-1, PUM