Western Blot - Anti-MUC1 Antibody [ARC0352] (A309097)
Western blot analysis of extracts of Rat lung, using Anti-MUC1 Antibody [ARC0352] (A309097) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Western Blot - Anti-MUC1 Antibody [ARC0352] (A309097)
Western blot analysis of extracts of Rat lung, using Anti-MUC1 Antibody [ARC0352] (A309097) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 1min.
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-MUC1 Antibody [ARC0352] (A309097) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using Anti-MUC1 Antibody [ARC0352] (A309097) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver cancer (negative control sample) using Anti-MUC1 Antibody [ARC0352] (A309097) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-MUC1 Antibody [ARC0352] (A309097) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human normal uterus (negative control sample) using Anti-MUC1 Antibody [ARC0352] (A309097) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.