Recombinant Anti-Mouse IgG2b Antibody [RM108] (A121332)

Name: Anti-Mouse IgG2b Antibody [RM108]
Brand: Antibodies.com
SKU: A121332
Price: 495.0 USD
Availability: InStock

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$495

Recombinant rabbit monoclonal [RM108] antibody to Mouse IgG2b for IP, ICC, IHC, Flow Cytometry, ELISA and WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

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Recombinant Antibody

Specifications

Name

Anti-Mouse IgG2b Antibody [RM108]

Description

Recombinant rabbit monoclonal [RM108] antibody to Mouse IgG2b.

Specificity

This antibody recognizes the Fc region of mouse IgG2b.

Applications

IP, ICC, IHC, Flow Cytometry, ELISA, WB

Dilutions

ELISA: 0.005-0.2 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml, WB: 0.10-5 µg/ml

Reactivity

Mouse

Cross Reactivity

There is no cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.

Immunogen

Mouse IgG2b.

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM108

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Mouse IgG2b Antibody [RM108] (A121332)

Western blot of non-reduced (-) and reduced (+) mouse IgG2b (20ng/lane), using 0.2µg/ml of Anti-Mouse IgG2b Antibody [RM108]. This antibody reacts to non-reduced IgG2b (~150 kDa) much stronger than the reduced ?2b form (~50 kDa).

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Anti-Mouse IgG2b Antibody [RM108] (A121332)

ELISA of mouse immunoglobulins shows Anti-Mouse IgG2b Antibody [RM108] reacts to the Fc region of mouse IgG2b. There is no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG2b Antibody [RM108] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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Anti-Mouse IgG2b Antibody [RM108] (A121332)

A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of Anti-Mouse IgG2b Antibody [RM108] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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