Mouse IgG1 - Isotype Control (A284022)

Mouse IgG1 - Isotype Control

Flow Cytometry, IHC-Fr

Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Mouse

Monoclonal

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine (Maslanka et al, 2012), ovine, porcine (Kapetanovic et al, 2012), equine (Jacks et al, 2007), canine (Maiolini et al, 2012) , lapine (Pakandl et al, 2008) and guinea-pig tissues.This reagent recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.