Anti-MMP9 Antibody [B10-7B9] (Biotin) (A279740)

Anti-MMP9 Antibody [B10-7B9] (Biotin)

Mouse monoclonal [B10-7B9] antibody to MMP9 (Biotin).

This antibody recognises matrix metalloproteinase 9 (MMP-9) also known as 92 kDa gelatinase and 92 kDa type IV collagenase. MMP-9 is a member of the zinc-dependent endopeptidases and is expressed in various cell types, including neutrophils, macrophages, fibroblasts and endothelial cell (Huang 2018). Expression of MMP-9 is induced by pro-inflammatory cytokines and is synthesized as a pre-proenzyme with a 19 amino acid N-terminal signal peptide. It can then be secreted by the cell into the extracellular environment where it is modified into the inactive pro-MMP9. At this point it can be activated by various proteases such as MMP-3, to generate the activated 82 kDa form. Activated MMP-9 can proteolytically cleave many extracellular matrix proteins including gelatin, collagen and elastin as part of extracellular matrix remodeling.Overexpression of MMP-9 has been observed in a range of malignant tumors and its’ extracellular matrix remodeling has been linked with mediating tumor invasion, metastasis, angiogenesis and regulating the tumor microenvironment (Huang 2018). MMP-9 has also been associated with other pathologies including autoimmune and cardiovascular diseases.The biotinylated Mouse anti Human MMP-9 antibody, clone B10-7B9 (MCA6197B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-9 antibody, clone I13-2E1 (MCA6196GA) as the capture antibody.

ELISA: 1 µg/ml

Human

E. coli derived recombinant fragment of human MMP-9 (Gly373-Pro456).

Mouse

Monoclonal

B10-7B9

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human MMP-9 antibody, clone B10-7B9, recognizes matrix metalloproteinase 9 (MMP-9) also known as 92 kDa gelatinase and 92 kDa type IV collagenase. MMP-9 is a member of the zinc-dependent endopeptidases and is expressed in various cell types, including neutrophils, macrophages, fibroblasts and endothelial cell (Huang 2018). Expression of MMP-9 is induced by pro-inflammatory cytokines and is synthesized as a pre-proenzyme with a 19 amino acid N-terminal signal peptide. It can then be secreted by the cell into the extracellular environment where it is modified into the inactive pro-MMP9. At this point it can be activated by various proteases such as MMP-3, to generate the activated 82 kDa form (Vandooren et al. 2013). Activated MMP-9 can proteolytically cleave many extracellular matrix proteins including gelatin, collagen and elastin as part of extracellular matrix remodeling. Overexpression of MMP-9 has been observed in a range of malignant tumors and its’ extracellular matrix remodeling has been linked with mediating tumor invasion, metastasis, angiogenesis and regulating the tumor microenvironment (Huang 2018). MMP-9 has also been associated with other pathologies including autoimmune and cardiovascular diseases.The biotinylated Mouse anti Human MMP-9 antibody, clone B10-7B9 (MCA6197B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-9 antibody, clone I13-2E1 (MCA6196GA) as the capture antibody.

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase-9, MMP-9

This product is for research use only. It is not intended for diagnostic or therapeutic use.