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Anti-MMP9 Antibody [B10-7B9] (Biotin) (A279740)

$435

Mouse monoclonal [B10-7B9] antibody to MMP9 (Biotin) for ELISA.

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Anti-MMP9 Antibody [B10-7B9] (Biotin)
Description
Mouse monoclonal [B10-7B9] antibody to MMP9 (Biotin).
Specificity
This antibody recognises matrix metalloproteinase 9 (MMP-9) also known as 92 kDa gelatinase and 92 kDa type IV collagenase. MMP-9 is a member of the zinc-dependent endopeptidases and is expressed in various cell types, including neutrophils, macrophages, fibroblasts and endothelial cell (Huang 2018). Expression of MMP-9 is induced by pro-inflammatory cytokines and is synthesized as a pre-proenzyme with a 19 amino acid N-terminal signal peptide. It can then be secreted by the cell into the extracellular environment where it is modified into the inactive pro-MMP9. At this point it can be activated by various proteases such as MMP-3, to generate the activated 82 kDa form. Activated MMP-9 can proteolytically cleave many extracellular matrix proteins including gelatin, collagen and elastin as part of extracellular matrix remodeling.Overexpression of MMP-9 has been observed in a range of malignant tumors and its’ extracellular matrix remodeling has been linked with mediating tumor invasion, metastasis, angiogenesis and regulating the tumor microenvironment (Huang 2018). MMP-9 has also been associated with other pathologies including autoimmune and cardiovascular diseases.The biotinylated Mouse anti Human MMP-9 antibody, clone B10-7B9 (MCA6197B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-9 antibody, clone I13-2E1 (MCA6196GA) as the capture antibody.
Applications
ELISA
Dilutions
ELISA: 1 µg/ml
Reactivity
Human
Immunogen
E. coli derived recombinant fragment of human MMP-9 (Gly373-Pro456).
Host
Mouse
Clonality
Monoclonal
Clone ID
B10-7B9
Isotype
IgG2a
Conjugate

Biotin

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human MMP-9 antibody, clone B10-7B9, recognizes matrix metalloproteinase 9 (MMP-9) also known as 92 kDa gelatinase and 92 kDa type IV collagenase. MMP-9 is a member of the zinc-dependent endopeptidases and is expressed in various cell types, including neutrophils, macrophages, fibroblasts and endothelial cell (Huang 2018). Expression of MMP-9 is induced by pro-inflammatory cytokines and is synthesized as a pre-proenzyme with a 19 amino acid N-terminal signal peptide. It can then be secreted by the cell into the extracellular environment where it is modified into the inactive pro-MMP9. At this point it can be activated by various proteases such as MMP-3, to generate the activated 82 kDa form (Vandooren et al. 2013). Activated MMP-9 can proteolytically cleave many extracellular matrix proteins including gelatin, collagen and elastin as part of extracellular matrix remodeling. Overexpression of MMP-9 has been observed in a range of malignant tumors and its’ extracellular matrix remodeling has been linked with mediating tumor invasion, metastasis, angiogenesis and regulating the tumor microenvironment (Huang 2018). MMP-9 has also been associated with other pathologies including autoimmune and cardiovascular diseases.The biotinylated Mouse anti Human MMP-9 antibody, clone B10-7B9 (MCA6197B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-9 antibody, clone I13-2E1 (MCA6196GA) as the capture antibody.
Synonyms
92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase-9, MMP-9
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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