Western blot analysis of rat liver showing detection of ~78 kDa MMP9 protein using Anti-MMP9 Antibody (A305207) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat Liver. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~78 kDa. Other Band(s): 64 kDa.
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