Anti-MMP12 Antibody (A11474)

Name: Anti-MMP12 Antibody
Brand: Antibodies.com
SKU: A11474
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to MMP12 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-MMP12 Antibody

Description

Rabbit polyclonal antibody to MMP12.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MMP12 (NP_002417.2).

Sequence

DEFWTTHSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADDIRGIQSLYGDPKENQRLPNPDNSEPALCDPNLSFDAVTTVGNKIFF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase-12, ME, MME, MMP-12

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-MMP12 Antibody (A11474)

Western blot analysis of extracts of A-549 cells, using Anti-MMP12 Antibody (A11474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunofluorescence - Anti-MMP12 Antibody (A11474)

Immunofluorescence analysis of A-549 cells using Anti-MMP12 Antibody (A11474) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-MMP12 Antibody (A11474)

Immunofluorescence analysis of NIH/3T3 cells using Anti-MMP12 Antibody (A11474) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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