Anti-MK2 Antibody (A82853)

Name: Anti-MK2 Antibody
Brand: Antibodies.com
SKU: A82853
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to MK2 for ELISA, WB and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-MK2 Antibody

Description

Goat polyclonal antibody to MK2.

Applications

ELISA, WB, IP

Dilutions

ELISA: 1:32,000, WB: 0.5-2µg/ml

Reactivity

Mouse

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 44-55, of human MK2.

Sequence

C-PQFHVKSGLQIK

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP-K2, MAPKAPK-2, MAPKAPK2, MK-2

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-MK2 Antibody (A82853)

MK2 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress GFP-tagged MK5 and incubated with Anti-MK2 Antibody (left) and Anti-MK2 Antibody (A82853) (right) at 0.5µg/ml.

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Western Blot - Anti-MK2 Antibody (A82853)

MK2 expression in MK2/MK3 double knockout mouse embryonic fibroblasts (MEFs) analyzed by western blot following immunoprecipitation (IP) using Anti-MK2 Antibody (A82852) (Lanes 3 + 4) and Anti-MK2 Antibody (A82853) (Lanes 5 + 6). Lanes 3 and 5 show MK2/MK3 expression rescued by retroviral transduction. Inputs for double KO and rescue MEFs were run in parallel (Lanes 1 + 2). Western blot was performed with a separate mouse-Anti-MK5 antibody.

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