Recombinant chimeric monoclonal antibody to Folate Binding Protein/FBP.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Rat, Mouse
Host
Chimeric
Clonality
Monoclonal
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 150 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE analysis of Mirvetuximab Biosimilar - Anti-Folate Binding Protein/FBP Antibody - Low endotoxin, Azide free (A323583) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Mirvetuximab Biosimilar - Anti-Folate Binding Protein/FBP Antibody - Low endotoxin, Azide free (A323583) confirms that the purity of this antibody is 100%.
Immobilized recombinant human FRa protein (His tag) at 2 µg/ml is bound by Mirvetuximab Biosimilar - Anti-Folate Binding Protein/FBP Antibody - Low endotoxin, Azide free (A323583). EC50 = 0.01123 µg/ml.
SKOV3 cells were stained with Mirvetuximab Biosimilar - Anti-Folate Binding Protein/FBP Antibody - Low endotoxin, Azide free (A323583) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.03238 µg/ml.
The endocytosis ratio of Mirvetuximab Biosimilar - Anti-Folate Binding Protein/FBP Antibody - Low endotoxin, Azide free (A323583) by human Fra HEK 293 increased with the increase of antibody concentration, and the Internalization Rate (%) 75% at antibody concentration of 55 ng/mL.
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